The Dhyana95 uses backside-illuminated sCMOS thinned chip technology to avoid light interference from the wiring layer, thereby increasing the pixel area and improving the photoelectric conversion rate.
At 560nm, the quantum efficiency of Dhyana 95 is 95%, which a front illuminated sCMOS camera cannot match, and is comparable to the best EMCCD camera.
The Dhyana95 read noise is only 1.45 electrons(peak), therefore the signal to noise ratio is significantly better than other sCMOS cameras, and when the incident photons are>3, there is a better performance than EMCCD based cameras
The 2" array can not only adapt to more optical interfaces and deliver a greater field of view, but also results in fewer lens switches to find the area of interest on the sample.
Under very weak laser illumination (< 0.002 kW cm-2 ), Dhyana 95 camera showed comparable fluorescent intensity to the 97%QE EMCCD camera with 100 EM gain. On the other hand, Dhyana 95 had similar background fluorescence noise when comparing to the 82%QE sCMOS camera, which has 1.0e-low read noise.
STORM stochastic optical imaging microscopy is one of the most widely used and the highest resolution super-resolution methods. It requires a camera to have the performance advantages of high quantum efficiency, high frame rate and low noise in the visible range. Its spatial resolution is determined by the single molecule localization accuracy and Nyquist resolution simultaneously.
Sensor size
2〃
Sensor model
G400 BSI (Backside-illuminated sCMOS)
Color/monochrome
Monochrome
Quantum efficency
95%@560nm
Effective no. of pixels
2048(H) x 2048(V)
Pixel size
11 x 11(μm)
Effective area
22.528× 22.528(mm)
Full well capacity
90,000e-
Frame rate
24fps@(2048x2048 via USB3.0)
Readout noise
1.45e-(Peak), 1.71e-(Median)
Shutter type
Rolling shutter
Exposure mode
Manual / Auto
Exposure time
0.021ms-10s
Cooling method
Peltier cooling
Cooling temperature
Forced air(Ambient at +25℃):-10 ℃
Dark current
3 electrons/pixel/s(0 ℃)(typ.)
1.5 electrons/pixel/s (-10 ℃)(typ.)
Dynamic range
86dB
Sub-array
available
External trigger mode
Standard/Synchronous/Global trigger
Trigger delay function
0-10s（1μs steps）
Trigger output
3 programable timing output (Exposure/Global/readout signal)
External trigger routing
SMA
Digital interface
USB3.0
SDK
Support
Bit depth
16 bit
Lens mount
T or C-mount
Power supply
12V/8A
Power consumption
50W
Camera size
120 x 119 x 121 (mm)
Parameter settings
White balance, Exposure, Contrast, Gamma, 3D denoise, Saturation, Flat fielding
PC software
Mosaic/LabVIEW/Matlab/Micromanager
Compatible system
Windows/Linux/Mac
Operating temperature
0-60℃
Operating humidity
10%-85% RH